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KMID : 0362320100350040285
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Journal of Korean Academy of Operative Dentistry
2010 Volume.35 No. 4 p.285 ~ p.294
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THE EVALUATION OF PERIODONTAL LIGAMENT CELLS OF RAT TEETH AFTER LOW-TEMPERATURE PRESERVATION UNDER HIGH PRESSURE
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Chung Jin-Ho
Kim Jin
Choi Seong-Ho
Kim Eui-Seong
Park Ji-Yong
Lee Seung-Jong
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Abstract
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The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at under pressure of 2 MPa), group 8 (low-temperature preservation at under no additional pressure), group 9 (low-temperature preservation at under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 (/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at under pressure of 2 MPa suggest the possibility for long term preservation of teeth.
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KEYWORD
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Periodontal ligament cell, MTT, WST-1, Viability, Low temperature preservation under pressure, Rapid freezing, Cryopreservation
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